A Crack in Creation 评价人数不足
读书笔记 Cracking the code
山谷修炼㍿

production of cas proteins in e. coli

Cas1: cut up e. coli DNA for phage DNA insertion

Cas6: slice long CRISPR RNA

RNA dection tool

Cas3: chop up target DNA in Type I system

Type II: precise clipping

S. thermophilius vs. S. pyogenes

Csn1 / Cas9

defining the minimal components: CRISPR RNA, tracrRNA and Cas9

chimeric RNA

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